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PubMed COVID-19 Clinical Care

20171 - 20180 of 44979 results found

Facile prepared microfluidic chip for multiplexed digital RT-qPCR test

Date
Wednesday, September 13, 2023 - 4:00 AM
Description
The chip-based digital polymerase chain reaction (PCR) is an indispensable technique for amplifying and quantifying nucleic acids, which has been widely employed in molecular diagnostics at both fundamental and clinical levels. However, the previous

Bisulfite and Nanopore Sequencing for Pseudouridine in RNA

Date
Wednesday, September 13, 2023 - 4:00 AM
Description
ConspectusNucleophilic addition of bisulfite to pyrimidine bases has been known for a half century, and the reaction has been in use for at least a quarter of a century for identifying 5-methylcytidine in DNA. This account focuses on the chemistry of